Journal: PLOS Genetics

Article Title: Functional characterization of eicosanoid signaling in Drosophila development

doi: 10.1371/journal.pgen.1011705

Figure Lengend Snippet: (A) Schematic diagram of prostanoid synthetic pathways. Multiple prostanoids are synthesized from arachidonic acid (AA) by different enzymes. (B) Drosophila enzymes orthologous to human prostanoid synthases were identified by phylogenetic analyses. Phylogenetic trees are shown in – . PGD, PGE, and PGF synthases are highly conserved in insects, although D. melanogaster lacks some of them. (C) Prostanoids detected in whole body extracts of pupae by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based lipidomics. AA was detected in intact pupae at 0–4 hours after pupation, whereas prostanoids were undetectable. PGD2, PGE2, and PGF2α were detected in pupae that were raised in AA-supplemented diet during larval development. Data shown are mean total amount of eicosanoids in the whole body per animal ± SEM. n = 4–5.

Article Snippet: In this system, the cells were injected into 50 μl of the assay medium containing 200 μM of a prostaglandin or a prostanoid analog (Cayman Chemical, PGD2, #12010; PGE2, #14010; PGF2α, #16010; U-46619, #16450; iloprost, #18215).

Techniques: Synthesized, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy